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Blood feeding behavior of dengue-2 virus-infected Aedes aegypti. As with isolation systems that use baby mice, viruses that are isolated frequently require many passages before a consistent cytopathic effect can be observed in the infected cultures. Molecular evolution and epidemiology of dengue-3 viruses. While Africa has not yet had a major epidemic of DHF, sporadic cases have occurred, with increased epidemic dengue fever, in the past 15 years. Phenotypic expression of genetic changes in the virus genome may include increased virus replication and viremia, severity of disease virulence , and epidemic potential. There is increased proliferation of reticuloendothelial cells in the bone marrow, spleen, lymph nodes, and lungs. This proactive surveillance system is designed to monitor disease activity during the interepidemic period, prior to epidemic transmission.

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